GO will establish a task force to campaign for a Brexit in the lead up to the referendum which will determine the UK's membership of the 28-strong bloc.
The group is being formed in response to frustrations with other anti-Brussels campaigns which are too focused on "party politics".
Around 2,000 people are expected to attend the launch event in Northamptonshire.
"We are all united in our commitment to work at grassroots level to get the United Kingdom out of the European Union."
Established campaigns Vote Leave and Leave.EU have spent months battling it out to win the designation of the Electoral Commission as the official "out" voice in the referendum.
For more detail :MPs unite against Brussels bureaucrats to form anti-EU Grassroots Out campaign
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